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KMID : 0880319970020010019
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Journal of Oriental Medicine
1997 Volume.2 No. 1 p.19 ~ p.24
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Matrix Degrading enzymes of u-PA , PAI-1 and nm-23 Gene expression administering complex prescription of Huoxue Huayu
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Gongshu Li
Hongsheng Lin/Leyu Wang/Zhenchun Bi/Chong Kwan Cho/Bingkui Piao
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Abstract
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Background : The complex prescription of Huoxue Huayu is effective anti-metastasis drug for lung cancer by the clinic, and further basic experiments showed that it decreased the activity Urokinase-type plasminogen activator (u-PA) and increased the active of its inhibitor PAI-1 so as to impede matrix degradation and inhibit metastasis. In addition, nm23 gene has also been closely related to the course of carcinoma matastasis.
Method: The trial involved 42 patients with stage I-III a lung carcinoma, 20 patients in the treated group, and 22 patients in the controlled group. All 20 patients in the treated group receives the complex prescription of Huoxue Huayu for 10 days preoperation. Using immunohistochemical assay, we observed u-PA, PAI-1 and nm23 expression in the tumor center, margin, stroma and normal lung tissue in lung carcinoma.
Results : In the treated group, the uPA-positive rates in the center, margin and stroma of the tumor were 60%(12/20), 55%(11/20), and 20%(4/20) respectively The PAI-1-positive rates were 95%(19/20), 95%(19/20), and 65%(13/20) respectively. In the controlled group, the uPA-positive rates were 63.64%(14/22), 50%(11/22), 31.82%(7/22), and PaI-positive rates were 59.09%(13/22), 77.27%(17/22), and 59.10%(13/22) respectively in the same tumor site. The tumor nm23-positive rates in the controlled group were 45.45%(10/22); but in the treated group, the tumor nm23-positive rates were 65%(13/20). In the controlled group, the expression of u-PAI-1 in tumor and stroma of No was higher than u-PA; The expression of u-PA in N2 was higher than No; and the expression of PAI-1 in N2 was less than No. In LNM-patients, the expression of PAI-1 was high and u-P low. On the contrary, LNM+patients showed high expression of u-PA and low expression of PAI-1. In the treated group, the expression of the margin and strom a in No was higher than u-PA(P<0.01), the expression of u-PA weakened and PAI-1 enhanced, which, however, showed no significance compared with N2 of the controlled group of with NO of the treated group. The result suggested that the relationship of expression between N2 and No in the treated group differed from the controlled. In the controlled group, the positive expression of the nm23 gene was higher in LNM-patients than in LNM=. In the treated group, the positive rate of nm23 was higher in LNM-patients than in LNM+. In the treated group, the positive rate of nm23 was higher than the controlled group, whether LNM-or not. However in both groups the cases were few and statistical analysis was not different.
Conclusion : In summary, the mechanism by which the prescription consisting mainly of Huoxue Huayu inhibits metastasis of lung carcinoma may be due to the impediment of enzyme degradation during metastasis.
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KEYWORD
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